Experimental Results and Discussion:

Dataset collection

By-products of 120 fresh farmed tilapia (oreochromus niloticus) were collected every month over a year at Kafrelsheikh Governorate, Egypt (One of the most important areas in the production of tilapia in the world). We collected fish byproduct under measured parameters (weight, Sex, length, water quality, ration). Then, they were minced and stored at −30 °C till use. The following steps shows the Enzymatic hydrolysis reaction process for preparing the data samples.

Preparation Phase

Analysis of Tilapia fish by-product and its hydrolysates powder
The contents of tilapia fish by-product and its hydrolysates were measured according to AOAC method28, The protein content was determined using kjeldal method. Moisture percentage was estimated with drying method. In addition, Ash content was measured by muffle furnace. Within our study, the protein hydrolysates were extracted with alcalase enzyme with appropriate PH and temperature29.
Amino acid sequence analysis
According to30, stacking and separating gel were prepared using gel buffer with percentage 4% and 16% respectively. Heating the sample mixture with the buffer till 90 °C for 10 min, then loading into specific wells. Protein standards (1.06 kDa to 26.6 kDa) were also performed on the gels. Fixing, staining and destaining solutions were mixed with gel, after electrophoresis then comparing the resulted protein bands with the standard ones31.

Tricine SDS-PAGE analysis

According to 30, we performed Tricine-SDS-PAGE by preparing gel buffer with 4% and 16% stacking and separating gel respectively, then fixing solution was added to gels. After that staining solution was added before the destaining solution. Comparing the resulted bands with standard protein bands.

 Measuring the ACE inhibition Activity:

(Adjust the protocol) will be added 

Results