• In the methodology, there is no reference of what the authors consider the low levels of ethanol; moreover, the statement that 20 mM EtOH is equivalent to 0.04 dg/L BAC lacks a reference or appropriate demonstration in larvae.
  • The term "Pavlovian fear conditioning" is inadequate to describe the aversive conditioning task used; in fact, what the authors are performing is avoidance conditioning, which has initial components of Pavlovian conditioning (CS-US) followed by instrumental conditioning (CS-CR).
  • In the procedure described for the task of appetitive conditioning it is mentioned that after training the fish were exposed to 5 trials of initiator light “on” (30-sec), or “off” (30-sec), however, in results is described that the fish were presented with the stimulus light 5-times (10-sec), interspersed with non-light presentations (10-sec).