ATP yield estimation using the obtained parameters
The model estimates the distribution of substrate to select a set of catabolic pathways to obtain the lowest residual error with respect to the measured metabolic product distribution during a cycle analysis. These fractions are combined with the ATP yield per catabolic reaction to obtain the overall yield of ATP on substrate (YATP,S). This yield is than combined with the biomass yields on glucose (Yx,s) observed in time (n=3) to obtain the biomass yield on ATP harvested (YX,ATP). An argumentation which catabolic routes were selected for both enrichments can be found in Appendix III.