Estimation of µmax from on-line data collected from bioreactors
A script was developed in Matlab version 2014 (MathWorks, Natick, Massachusetts, USA) to estimtate the growth rate over each SBR cycle. This script is based on determining the last moment of base dosage in a cycle, which indicates the end of the fermentation, and thus the growth. This method is explained in detail in the supplementary information section published previously (Rombouts et al., 2019). The maximum biomass-specific substrate conversion rate (qsmax) is calculated using µmax and Yx,s using the Herbert-Pirt equation (Pirt, 1965) and neglecting maintenance.