Parameter estimation of kinetics of the enrichment cultures
using minimisation of residual error
To estimate the kinetic parameters of the enrichments and to derive the
stoichiometry of the process a kinetic model was built. The uptake rate
was modelled using Monod equations and no biomass decay or pH inhibition
was considered. The saturation constant of the Monod equations for the
different microorganisms was assumed to be 0.1 g/L. It is assumed that
there is a microbial group degrading glucose and other degrading lactate
(only in the complex medium enrichment). In this last case, the measured
biomass was divided between glucose degraders (65%) and lactate
degraders (35%), based on the derived stoichiometry and the ATP yield
on the substrate corresponding to that stoichiometry. Parameter
estimation was performed following the method proposed by González-Gilet al. (Gonzalez-Gil et al., 2018) and is further explained in
Appendix II.