N-glycan Structure Determination
We performed glycan structure profiling of R27T. Herein, two strategies
for comprehensive N-glycan profiling, fluorescence profiling by
WAX/HILIC-HPLC, in which an HPLC database is publicly available
(Campbell, Royle, Radcliffe, Dwek, & Rudd, 2008; Royle et al., 2008;
Royle, Radcliffe, Dwek, & Rudd, 2006), and MALDI-MS, were employed in
this study.