2.2 Medium
The liquids such as MS, ½ MS, B5 (as Conventional culture media) and water were used to evaluate the performance of the mist generation system. The culture media were prepared according to the specific protocols, including macro, micro, vitamins, and iron components, with 15gr of sugar per liter and an adjusted pH of 5.8. Due to possible contamination, the culture media was autoclaved at a temperature of\(121\ C\ \)and pressure 1 bar to prevent any undesirable changes of the physicochemical properties. Sufficient medium was supplied and stored in the refrigerator during the experiment to prevent any contamination.