The materials and methods section should be brief but sufficient to allow other investigators to repeat the research (see also Policy Concerning Availability of Materials). Reference should be made to published procedures wherever possible; this applies to the original description and pertinent published modifications. The sex of subjects should be stated. If the sex of subjects is unknown, this should be stated. All companies from which materials were obtained should be listed. If materials were obtained from an individual, an affiliation for that individual should be listed. A manuscript that presents only a theory may omit the Materials and Methods section.

Experimental Design

For the control group, the crayfish remained in conditions as similar as possible as those in the treatment group, with the exception of the imidacloprid added to those in the treatment group. For the treatment group, 5 doses of treatments were added to each crayfish, and then the change in action potentials was recorded for the following 60 seconds. Each treatment consisted of 1 mL of an imidacloprid solution with a concentration of 10ng per liter. As a result of the experimental design, the concentration of imidacloprid increased as each treatment was added.