2.6 Statistical analysis
Each experiment was carried out in triplicate unless otherwise specified. All results are presented as the mean ± standard deviation (SD). The experimental data were analyzed using GraphPad Prism software, version 6.0, and Microsoft Excel. For comparisons among more than two groups, ANOVA (one-way and two-way) and the post hoc Tukey test. To determine the significance between two groups, Student’s t-tests were performed. Statistical significance was set at a p-value ≤ 0.05.
3 RESULTS AND DISCUSSION