Decellularized scaffolds are used for reconstruction of damaged tissues and organs because they continue to have their natural three-dimensional ECM framework and composition which is responsible for adhesion and proliferation of cells. The main focus of this study was to explore the decellularization property of soap nut pericarp extract (SPE), an herbal detergent, for development of decellularized tubular esophageal scaffold of caprine origin for clinical use and esophageal tissue engineering research. For this 2.5, 5.0 and 10% concentrations of SPE were used for decellularization of caprine esophageal tissues. Histological and scanning electron microscopic (SEM) analysis of tissue samples processed in 5% SPE confirmed complete decellularization with preservation of ECM’s microarchitecture and thus used for further characterization via DNA quantification, SDS-PAGE, mechanical tensile testing, Fourier Transform Infrared (FTIR) spectroscopy and, cytocompatibility assay. DAPI stained decellularized tissues revealed complete removal of nuclear components, verified by measurement of DNA content and SDS-PAGE. Tensile strength of the decellularized scaffolds decreased non-significantly (P > 0.05) as compared to native tissues. Seeded scaffolds showed growth and attachment of primary chicken embryo fibroblasts (P-CEFs) over and within the decellularized scaffolds. It was concluded that 5% SPE may replace the toxic biological detergents and other chemicals used for preparation of biocompatible decellularized caprine esophagus scaffolds.