Rationale, aims and objectives: An outbreak of HAdV-7 infection in a tertiary hospital that led to a death case and nosocomial infection is described in the report. 14 confirmed cases (8 laboratory-confirmed cases and 6 silent-infection cases) were identified. Methods: The epidemiology investigation was carried out and 75 nasopharyngeal swab specimens were collected. Extraction and detection of viral nucleic acids and virus isolation were done to confirm the pathogenic factor. Results: The overall attack rate of this outbreak was 18.7%. 14 samples of 75 specimens were positive for HAdV-DNA, the virus strains belonged to HAdV7 and had high homology with the predominant HAdV7 strain ( HAdV7 0901HZ/ShX/CHN/2009 GU230898), which was isolated in 2009 from Hanzhong, Shaanxi. Conclusion: There were no a standard fever clinic in the hospital and no strict infection control procedures when caring for the patients, which were the main causes of the outbreak. Setting up a standard fever clinic, improving diagnostic capabilities for rapid detection of HAdV infection, isolating the infection sources as soon as possible and adopting infection control precautions strictly were necessary to prevent nosocomial infections.