Growth inhibition effects of NCTD on cancer cell lines and LO2 cells
To determine whether NCTD has a cytotoxic effect on human cancer cells, we assessed the viability of human cancer cell lines (non-small lung cancer cell line A549, breast cancer MCF-7 cell line , hepatocellular carcinoma BEL-7402 cell line, nasopharyngeal carcinoma CNE-2 cell line) and human hepatic cell line LO2. Cells were exposed to different concentrations of NCTD for 72 h as revealed in Fig. 1B. NCTD showed significant proliferation inhibition and cytotoxic effects on these cancer cell lines, and showed moderate cytotoxic effects on normal LO2 cells. These data indicate that NCTD strongly inhibits tumor cell proliferation in a dose- and time-dependent manner. After 72 h of cell exposure, NCTD inhibited A549 cell activity in a dose-dependent manner with an IC50 value of 13.1 μM (Table 1). In addition, the clone formation test showed that NCTD significantly inhibited the cloning ability of A549 cells (Figure 1C).
fig. 1
table 1