NCTD induced apoptosis and reduced the mitochondrial membrane potential
In order to further study the mechanism of NCTD-induced cell proliferation inhibition, flow cytometry was used to analyze the changes in the apoptosis rate of cells after exposure to different concentrations of the compound NCTD. As shown in Figures 3A and 3C, after NCTD treatment of tumor cells, the percentage of A549 cells’ apoptotic rate increased from 4.0% to 40.1%, indicating that the rate of A549 cell apoptosis increased with the increase of NCTD
Depolarization of mitochondrial membrane potential (ΔΨm) is the key to cell death. Therefore, we used JC-1 staining to examine changes in mitochondrial membrane potential. As shown in FIG. 3B, as the NCTD concentration increased, Δ 降低 m in A549 cells gradually decreased. As shown in FIG. 3D, compared with the control group, cells treated with 20 μM and 40 μM NCTD for 24 hours both showed high green and low orange fluorescence under the fluorescence microscope, while the control group detected high green and high orange fluorescence.
fig.3.