Microfabrication
The geometry of the microfluidic device was designed using a CAD suite
(Layout Editor, Juspertor UG, Unterhaching, Germany) and fabricated in
polydimethylsiloxane (PDMS, Sylgard 184, Dow Corning, Midland, MI),
based on the previously published model (Gori et al., 2016), through the
same two-layer soft-lithographic process to pattern the cell culture
microchamber together with the transport channels and the
endothelial-like barrier, respectively.