Microfabrication
The geometry of the microfluidic device was designed using a CAD suite (Layout Editor, Juspertor UG, Unterhaching, Germany) and fabricated in polydimethylsiloxane (PDMS, Sylgard 184, Dow Corning, Midland, MI), based on the previously published model (Gori et al., 2016), through the same two-layer soft-lithographic process to pattern the cell culture microchamber together with the transport channels and the endothelial-like barrier, respectively.