To confirm whether different Pi application levels affect the P content in leaves, the Pi and organic phosphate (Po) content in the leaf blade were determined per unit of fresh weight (F.W.) and leaf area. The Pi content increased with increasing Pi application, and Po content also increased, but to a lesser extent (Table 1). These results indicated that Pi is accumulated in leaves, depending on the Pi concentration, and P toxicity can be caused in WT rice plants growing under Pi conditions higher than the control-Pi conditions.