Lipid hydroperoxide quantification
Lipid hydroperoxide (LOOH) in rice leaf blade was quantified by using
Spy-LHP (Dojindo, Japan) (Chan et al., 2012). A leaf blade was
homogenized in the extraction buffer [Pottasium-phosphate buffer (pH
7.0). 1 mM EDTA, 12.5% (v/v) Glycerol], and the resulting leaf
extract was incubated with 2.7 µM Spy-LHP for 30 min on ice. After
centrifugation, the supernatant was monitored for fluorescence emission
(RF-1500, Shimazu). The excitation and emission wave lengths were 520
and 540 nm, respectively.