Thepmf and gH+ were similar
between the control-Pi and low-Pi plants, butV H+ was slightly lower in the
low-Pi plants than in the control-Pi plants (Figure S2). In contrast,
the 3.0 mM Pi plants showed lower pmf ,
gH+, andV H+ than the control-Pi plants
(Figure S2). Both gH+ andVH+ showed linear responses to
Ci in a manner similar to A , Y(II), and Y(I) (Figure 2e, f and
Figure S1a). These results indicated that both pmf generation by
PET reaction and ATP synthesis are suppressed under 3.0 mM Pi conditions
(Takagi et al., 2016b, 2017). These observations indicated that
excessive Pi accumulation suppressed photosynthesis over a wide range of
Ci levels, and this phenomenon was different from that caused by low Pi
application in rice plants.