Primer design and relative quantification of gene expression
Gene-specific primers were designed using Oligo7 software (Rychlik,
2007); on Cucumis sativus L. gene sequences at the National
Center for Biotechnology Information (NCBI;
www.ncbi.nlm.nih.gov). The primer
sequences and their amplicon characteristics for each gene are presented
in Table1. The specificity of primers designed was confirmed by
performing the PCR reaction on synthesized cDNA and genomic DNA (To
confirm the accuracy of the PCR).