4. CONCLUSION
The present study concluded that medium supplemented with combination of 1mg/L BAP and 1mg/L 2,4-D had best callus responses from leaf disc explant for all Jatropha accessions. The maximum shoot regeneration (66.67%) from callus with 10.13 number of shoot was obtained from Shewa Robit accession in MS medium fortified with TDZ (0.5 mg/L) and IBA (0.1mg/L). However, shoot regeneration competence from the callus can differ depending on the type of accessions and plant growth regulators used. The callus induction rates obtained in this study were highest in comparison of earlier reports. Generally, the callus induction protocol thus developed can be used for the improvement of Jatropha through biotechnological tools.