2.2. Growth Regulators Stock Preparation
The Plant Growth regulators (PGRs) used for the study were the cytokinin, 6-benzyl aminopurine (BAP) and Thidiazuron (TDZ), and the auxins, 2, 4-dichlorophenoxyacetic acid (2,4-D) and indole-3- butyric acid(IBA). All PGRs stock solutions were prepared by weighing and dissolving the powder in distilled water at the ratio of 1 mg/ml. To begin the dissolving process, the powdered crystal of the PGRs was first weighed and dissolved in 3-4 drops of 1N NaOH and 1N HCl based on the type of PGR (NaOH for auxins and HCl for cytokinin). Then, the volume was adjusted by adding distilled water. Finally, growth regulators’ stock solutions were stored in a refrigerator at a specific temperature for each growth regulator.