2.1. Planting Material
The seed of three Jatropha accessions were collected from Amhara and Oromia region of Ethiopia and used for these tissue culture experiments (Table 1). The seeds were germinated on growth trays containing sterilized combination of soil, sand and manure in the ratio of 2:1:1, respectively and kept in the greenhouse condition of Holeta Agricultural Research center (HARC). They were watered thrice a week using a spraying can. After three weeks, the seed that germinated was transplanted into pot containing sterilized soil and kept as mother stock plant. After three months of growth (Fig.1); very young, health and vigorous part of the plant (nodal segment) was collected and used as a source of explants. The experiment was conducted at Plant Biotechnology Laboratory of Holeta Agricultural Research Center (HARC) 45 km West of Addis Ababa, Ethiopia.