2.3. Culture Medium Preparation
Culture medium was prepared by taking the proper amount of MS basal medium (Murashige and Skoog, 1962) stock solutions (mg/L) (Annex 1). Full-strength of MS with 30g/L of sucrose as carbohydrate source (w/v) for Callus induction and Shoot proliferation were used. The pH of the medium was adjusted to 5.8 (using 1N NaOH and 1N HCl) after addition of the growth regulators. Gerlite (2.5g/L) was added as a gelling agent after the volume and pH of the medium was adjusted. The media was sterilized by autoclaving at a temperature of 121°C with a pressure of 15 psi for 15 min and stored at room temperature.