Confirmation and transgene expression analysis of transgenic
lines
Genomic DNA was isolated from the leaves of putative barley transgenic
plants by using DNA express solution as per manufacturer’s instructions
(Himedia). Primers amplifying gene of interest TaHSFA6b(TaHSFA6b Full F -R ), and selection marker gene hygromycin
(HPT Full F -R ) were used to confirm their integration in
barley transgenic lines (Supplementary Table 1). Transgene expression ofTaHSFA6b was analyzed by RT-PCR in T1 generation of progeny of
transgenic lines.