1.3 Biopanning
Following the amplification and infection of the bacteriophage viruses
in the bacteria, the obtained phages are used for the biopanning
process. The process involves introduction of the libraries to the
target (cell, antigen, receptor or other proteins) bound onto the solid
surface. Unbound phages are washed and removed whereas, bound ones are
eluted, and the results are analyzed by the means ELISA (enzyme linked
immunosorbent assay). In order to obtained high affinity and specific
phages, 4-5 rounds of panning are performed using the phages obtained
from the preceding rounds [6].
This review is designed to highlight some of the significant
applications of phage display in the field of medical sciences,
implemented as a tool for diagnostic and therapeutic medicine.