Conclusion and future aspect
Up to 80 monoclonal antibodies, extracted from phage display technology have entered clinical trial stages and 7 antibodies are approved products. Lately, it has been considered as a gold-standard for the isolation of monoclonal antibodies due to its robust nature and high stability. Phages can bear harsh environmental conditions such as exposure to ultraviolent radiation, extreme temperature and pH and proteolytic enzymes [82]. In the field of medicine, this technology has been extensively used for the detection of biomarkers, drug delivery and analyzing molecular interactions. To the date, the method is performed in several research laboratory, focusing on clinical researches. However, among some of the limitations of the technique; the requirement of the library and the discovery of only the fraction of molecule (example: scFv or Fab) are the most cumbersome ones [82].
Nonetheless, the development of this technique, in the field of biotechnology and immunology, has made several investigations easier and has also allowed scientist and researches to come up to the better conclusions.