1.3 Biopanning
Following the amplification and infection of the bacteriophage viruses in the bacteria, the obtained phages are used for the biopanning process. The process involves introduction of the libraries to the target (cell, antigen, receptor or other proteins) bound onto the solid surface. Unbound phages are washed and removed whereas, bound ones are eluted, and the results are analyzed by the means ELISA (enzyme linked immunosorbent assay). In order to obtained high affinity and specific phages, 4-5 rounds of panning are performed using the phages obtained from the preceding rounds [6].
This review is designed to highlight some of the significant applications of phage display in the field of medical sciences, implemented as a tool for diagnostic and therapeutic medicine.