Conclusion and future aspect
Up to 80 monoclonal antibodies, extracted from phage display technology
have entered clinical trial stages and 7 antibodies are approved
products. Lately, it has been considered as a gold-standard for the
isolation of monoclonal antibodies due to its robust nature and high
stability. Phages can bear harsh environmental conditions such as
exposure to ultraviolent radiation, extreme temperature and pH and
proteolytic enzymes [82]. In the field of medicine, this technology
has been extensively used for the detection of biomarkers, drug delivery
and analyzing molecular interactions. To the date, the method is
performed in several research laboratory, focusing on clinical
researches. However, among some of the limitations of the technique; the
requirement of the library and the discovery of only the fraction of
molecule (example: scFv or Fab) are the most cumbersome ones [82].
Nonetheless, the development of this technique, in the field of
biotechnology and immunology, has made several investigations easier and
has also allowed scientist and researches to come up to the better
conclusions.