2.4 Preparation of curcumin solution
The tested curcumin powder was first dissolved in 10 ml of pure ethanol and then solution diluted using deionized water. The amount of curcumin was calculated according to its mass and purity, and accordingly, the available solution for the study was made with different concentrations in a volume of 500 ml, and finally, 100 ml of it was considered as a statistical population.