Amplicon library preparation and sequencing
Samples of 190 Polyplax serrata , 14 Hoplopleura
acanthopus, and 2 Hoplopleura edentula were collected from
different rodent species and populations from 2014-2018. Details on
sample origin, including the sampling locations, GPS coordinates, and
rodent host species, are provided in SupplementaryData2. DNA templates
were extracted from each individual using QIAamp DNA Micro Kit (Qiagen,
Hilden, Germany). Two independent multiplexed 16S rRNA gene amplicon
libraries were built for each of the louse genera. Detailed description
of the library designs and MiSeq sequencing is provided in
SupplementaryInformation/SupplementaryText. Amplicon data for the lice
microbiomes (demultiplexed, quality filtered and merged) are available
at https://www.ebi.ac.uk/ena/data/view/PRJEB35541.