Phylogeny
In both phylogenies (the 16S rRNA gene and the multigene) the twoNeisseria -related symbionts cluster as sister taxa on a long
common branch, placed firmly within Neisseriales with high nodal
support. In the more robust multigene analysis the pair branches as an
isolated offshoot at the base of Neisseriaceae (Figure 1). The analysis
of 16S rRNA gene, for which a broader taxonomic spectrum is available,
revealed additional close relatives which could not be included into the
“multigene matrix” due to lack of data
(SupplementaryInformation/SupplementaryFigure1; detail in Figure 1). All
of them have been described as “Uncultured bacterium ” from
human and insect samples (Oteo et al., 2014). In contrast to the
“multigene matrix”, the “16S matrix” placed the pair ofNeisseria -related symbionts into a monophyletic cluster together
with Snodgrassella .