Estimation of seed yield
8-week-old wild-type Col-0 and fum2.2 plants were kept in control conditions or were cold treated for 1 week as described above. Plants were then moved to a growth chamber with a 16-hour daylength, 20oC, to trigger flowering. After a further 8 weeks, the number of siliques per plant was counted on 6 plants for each treatment group and the number of seeds silique pod was estimated for 10 individual siliques per plant. Total seed yield was estimated as the product of silique number and mean seeds per silique.