Effect of Species and phylogeny on the entire GSL matrix and
phytochemical diversity.
Across all Cardamine species we found 51 GSL compounds: 22
aliphatic-GSLs, 6 aromatic-GSLs, 3 indole-GSLs, and 20 unknown GSLs
(Table S2). Plant species differed significantly in the identity and
amount of GSLs produced (Fig. 1; PERMANOVA: F13, 64 =
16.956, p = 0.001), but the difference in GSLs’ profiles was not
correlated with phylogenetic distance across species (Fig. 1, Mantel
test: r = 0.15, p = 0.3). In addition, we found significant differences
among species in all the seven indices of GSL diversity (Table S3).