Auditory electrophysiology
Auditory brainstem response (ABR) was used to evaluate the hearing function of wild-type and mutant pigs, as described by Guo et al(Guo et al., 2015). Briefly, the ABRs were evoked with clicks and tone pips at 1, 2, 4, 8, 16, 24, and 32 kHz. The response was amplified, filtered, and averaged using the intelligent hearing system (TDT, USA). And the ABR threshold was determined by visual inspection.