Animals
Bama pigs were maintained at the Center of Experimental Animal in the Army Medical University and allowed ad libitum access to a commercial pig diet (nutrient levels according to the United States National Research Council) and clean water throughout the experimental period. The generation of mutant pigs has been described previously(Hai, Cao, et al., 2017). Briefly, the G0 pigs (10-month-old, male) were injected three times intravenously with ENU at a dose of 85 mg/kg at 1-week intervals to generate random genome-wide mutations in germ cells. After a recovery period of one month, the treated G0 boars were mated with wild-type sows to produce G1 progeny. G1-016105 used in this study was one of the dominant progenies found in G1 and was crossed with ten wild-type females for inheritance testing.