Proliferation Assay
Cell proliferation assay is a simple in vitro assay used in establishing the activity of a variety of proteins including IL-22 (Cai et al, 2010). Interleukin-22 is known to initiate tissue preservation and repair. Therefore cell proliferation is expected to be enhanced in the presence of cfIL-22. Normalized to the no treatment control, Figure 3 demonstrates cfIL-22 concentrations from 100 to 1500 ng/ml increased CCO cell proliferation in comparison to pBK plant background control.