Proliferation Assay
Cell proliferation assay is a simple in vitro assay used in
establishing the activity of a variety of proteins including IL-22 (Cai
et al, 2010). Interleukin-22 is known to initiate tissue preservation
and repair. Therefore cell proliferation is expected to be enhanced in
the presence of cfIL-22. Normalized to the no treatment control, Figure
3 demonstrates cfIL-22 concentrations from 100 to 1500 ng/ml increased
CCO cell proliferation in comparison to pBK plant background control.