2.9 Macroscopic evaluation of wounded tissues
For measurement of wound closure percentage, wounds were photographed at 0, 3, 7, 10, 14, and 21 d post-wounding and analyzed by using ImageJ software. The wound area was measured by the limits of grossly evident epithelialization. The wound closure percentage at each time point was derived by the following formula: [(Initial wound size-current wound size)/initial wound sizeƗ100].