2.4. Cell Migration Assay
An in vitro scratch wound healing model was used to assess cell
migration. HaCaT cells were plated in a 12-well plate and grown to
complete fusion. Next, they were scraped in a straight line with a
100-μL pipette tip to create a uniform cell-free zone and rinsed three
times with PBS. Then the cells were treated with normal medium or medium
containing EGF or RHC/EGF. The images of the cells at the beginning and
at regular intervals during cell migration to close the scratch were
captured and compared through quantifying the migration rate of the
cells.