Promoter clone and tissue-specific expression by GUS strain inA. thaliana
Because LaCYP71D582 and LaTPS7 showed a relationship of successive catalysation, we further investigated the precise expression pattern of these genes. Thus, a 1299-bp promoter of LaTPS7 and a 1434-bp promoter ofLaCYP71D582were obtained via FPNI-PCR and designated asPro -LaTPS7and Pro -LaCYP71D582 . Both of these promoters not only contained the basic promoter elements, but also harboured several regulatory elements predicted by PlantCARE (Figure S6, S7). We then constructed A. thaliana lines transformed with each gene promoter-GUS fusion vectors. Strain assay ofLaTPS7showed that it was expressed in flowers, siliques, trichomes and leaves. Its expression in leaves, in particular, exhibited a wound-induced pattern (Figure 6a). In other words,LaTPS7 was only highly expressed in leaves after a leaf had been injured, similar to the response observed in leaves being attacked by herbivores. LaCYP71D582 showed a constitutive expression pattern in flowers, siliques, trichomes, and leaves (Figure 6b). This response was similar to that of LaTPS7 , but expression of LaCYP71D582 did not show a wound-induced pattern.