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From graphite to laccase biofunctionalized few-layer graphene: a “one pot” approach using a chimeric enzyme
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  • Ilaria Sorrentino,
  • Ilaria Stanzione,
  • Yannig Nedellec,
  • Alessandra Piscitelli,
  • Paola Giardina,
  • Alan le goff
Ilaria Sorrentino
CNRS
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Ilaria Stanzione
Universita degli Studi di Napoli Federico II Complesso Universitario di Monte Sant\'Angelo
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Yannig Nedellec
CNRS
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Alessandra Piscitelli
Universita degli Studi di Napoli Federico II Complesso Universitario di Monte Sant\'Angelo
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Paola Giardina
University of Naples
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Alan le goff
CNRS
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Abstract

A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer-graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced biofunctionalized FLG was characterized by electrochemistry and Raman spectroscopy and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications.