Fatty Acid Composition
Fatty acid methyl esters were prepared according to the American Oil
Chemists’ Society (AOCS) method Ce 2-66, and analyzed using an Agilent
Acme 7890B gas chromatograph (Santa Clara, the United States) equipped
with a flame ionization detector and capillary chromatographic column
BPX70 (60 m, 0.25 mm id and 0.25 mm film thickness) according to AOCS
method Ce 1-91 (15). Injection was performed at a split ratio of 1:100,
the detector and injector temperature was 270 and 250 °C, respectively.
The column was run isothermally at 198 °C, the carrier gas was nitrogen
and the column head pressure was 11.79 PSI.