Statistical analysis
The measurement data of the control, EB, and C-EB groups were compared
using Student’s test. Counting data were tested by a chi-square test and
Fisher’s exact probability method. SPSS 22.0 software (SPSS, Inc,
Chicago, IL, USA) was used for statistical analysis of the clinical
data. The Shannon, Simpson, Goods Coverage, Observed Species, and Chao1
indices curves were drawn to evaluate the depth of sample sequencing and
the diversity of intestinal flora (α - diversity) using Qiime process
software(Qiime version 1,University of Colorado, Colorado, USA) and R
language was used to draw the graph. For the operation classification
unit (OTU) table, Qiime was used to generate a species distribution map
at phylum level, and R language was used to draw the map. The Wilcoxon
rank sum test was used to analyze the significant differences among the
three groups of samples and the P value was calculated. Anosim
(company, city, state, country) was used to test the differences between
and within groups. A P < 0.05 has statistical
significance.