Statistical analysis
The measurement data of the control, EB, and C-EB groups were compared using Student’s test. Counting data were tested by a chi-square test and Fisher’s exact probability method. SPSS 22.0 software (SPSS, Inc, Chicago, IL, USA) was used for statistical analysis of the clinical data. The Shannon, Simpson, Goods Coverage, Observed Species, and Chao1 indices curves were drawn to evaluate the depth of sample sequencing and the diversity of intestinal flora (α - diversity) using Qiime process software(Qiime version 1,University of Colorado, Colorado, USA) and R language was used to draw the graph. For the operation classification unit (OTU) table, Qiime was used to generate a species distribution map at phylum level, and R language was used to draw the map. The Wilcoxon rank sum test was used to analyze the significant differences among the three groups of samples and the P value was calculated. Anosim (company, city, state, country) was used to test the differences between and within groups. A P < 0.05 has statistical significance.