Example of a properly written protocol step with an annotation:

3. Plate 1 × 106 cells/ml in R10 medium with 10 nM OVA257-264SIINFEKL peptide in individual wells of a 24-well or 6-well plate to activate cells.
Usually during the first 24 h, cell numbers will decrease by 25%. Please recount the cells after the incubation to make sure you use the correct amount in the following step.