INTRODUCTION

Briefly introduce the purpose of the method, what problem(s) it addresses, and what data can be obtained by executing the protocols. Most of the background literature should be cited in the Commentary section (see below). The final paragraph of the introduction should, in sentence form, list all the protocols in the document and provide a brief summary of each one.
STRATEGIC PLANNING (optional)
Occasionally a method is sufficiently complex that a Strategic Planning section is required. This section describes, in paragraph form, various procedural options that are essential to know before starting the protocol(s) below.