Materials

Provide an EXHAUSTIVE list, organized in two sections - the first consisting of reagents, solutions, and starting samples or test organisms/cells, and the second consisting of hardware and instruments (e.g., glassware, disposables, microscopes, centrifuges) used for this protocol, in the order in which they are introduced in the protocol steps below. Names of complex solutions (more than 3 components) and reagents should be listed here – with the recipe details provided in the Reagents and Solutions section (the components of those recipes are NOT listed here). Please ensure that enough detail for each material is added such that someone could obtain the exact material listed – depending on the material this might be supplier information, product numbers, CAS numbers, antibody clone numbers, RRIDs, etc.