HEK 293T cells (ATCC, cat. no. CRL-3216, RRID:CVCL_0063)
Protocol steps — Step annotations:
Numbered protocol steps describe the how to perform the protocol. Use the active voice (start each sentence with a verb such as weigh, place, measure) and include sufficient detail that the procedure can be successfully completed on the first attempt. Provide any pointers, additional information or explanation for a protocol step as an annotation, directly below the step to which it applies and in italics. Not every step requires an annotation, but authors are strongly encouraged to add annotations to enrich the instructions.