Example of a properly written protocol step with an annotation:
3. Plate 1 × 106 cells/ml in R10 medium with 10 nM
OVA257-264SIINFEKL peptide in individual wells of a 24-well or 6-well
plate to activate cells.
Usually during the first 24 h, cell numbers will decrease by 25%.
Please recount the cells after the incubation to make sure you use the
correct amount in the following step.