3.3. The increased mitochondrial fission in the kidney of KK-Ay
mice was rescued in the Empa group.
To examined the change of mitochondrial dynamics in diabetic
environment, we detected the
mitochondrial fission related
protein such as Drp1,
Drp1S616
and DRP1S637 and the pro-fusion protein Mfn2. Here we found no
significant difference in Drp1S616 and DRP1 between the KK-AY group and
NC group, but the DRP1S637 decreased notably in the KK-AY
group (Fig. 2A, E, F).
Meanwhile, we found an obvious
increase of mito-Drp1 in KK-AY group which verified the transfer of Drp1
from cytosol to mitochondria during the mitochondrial fission. The
changes of DRP1S637 and mito-Drp1 in KK-AY group were reversed in Empa
treated mice significantly (Fig. 2 C, E). There was no remarkable change
of MFN2 was found among the three groups (Fig. 2A, G).