During signaling, NFkB pathway often intersects with the mTOR pathway in a variety of ways. (Oeckinghaus, Hayden, & Ghosh, 2011; Trocoli & Djavaheri-Mergny, 2011). To understand whether the increase in NFKB inhibitor gene expression in PC corals is potentially related to modulations in NFkB/mTOR crosstalk after preconditioning, we analyzed the gene expression profile of pa-mTOR in PC and NPC corals during heat stress response (Figure S7). While the expression profile is dynamic over time, it does not vary between PC and NPC corals suggesting that NFkB pathway is affected in an mTOR-independent manner during thermal preconditioning in P. acuta.