Figure S5. Gene expression of pa-GFP in corals used in venetoclax experiment at 5 days. There is a disputable difference between Preconditioned (PC) and non-preconditioned (NPC) corals in venetoclax experiment but considering that we see a significant difference in bleaching rate of PC corals with and without venetoclax treatment, but not in pa-GFP gene expression between these two groups of corals, the difference between PC and NPC cannot cause a misinterpretation of bleaching rate microscopy data. Thus, along with the data in Figure S4, we can conclude that the observed shift in symbiont to host fluorescent ratio between PC ven+ and PC ven- corals is not likely caused by an increase in GFP gene expression during the treatment.
Figure S6