Figure S1 Expression of pHSF1, the major transcription factor of heat shock protein family . Expression of pHSF1 relative to control at time 0h. Control samples are depicted with dark grey dotted line, non-preconditioned (NPC) with light grey dashed line and preconditioned (PC) with black solid line. ΔΔCt method was used to calculate the relative expression and the ratios. Graph shows mean values with standard error bars. * marks significant (p ≦ 0.05) difference between PC and NPC calculated using one-way ANOVA with Tukey’s post hoc test for each timepoint individually, n = 10.