For Bcl-2 inhibition, fragments were exposed to 5-day acute heat stress at 32°C as described above. PC and NPCfragments were treated with 1μM venetoclax for the first 6 hours of the acute heat stress and then placed to venetoclax-free sea water (32°C) for the rest of the experiment. Two of seven colonies were excluded from statistical analysis because fragments did not survive to day 5 of heat stress.