Figure S5. The GFP and chlorophyll-derived autofluorescent signals normalized to area size analysis shows that during the venetoclax treatment experiment, it is the red signal that contributes the most to the changes in host to symbiont signal ratio differences between treatments. In other words, it is the loss of the Symbiodinium signal, not the increase in the host signal that drives the changes in symbiont: host signal ratio observed during heat stress experiments in P. acuta (Figure 1 and 4). The differences between PC and NPC corals with and without venetoclax are of particular interest in this figure. While the host signal remains relatively stable, it is the symbiont signal that decreases in all the corals except for PC coral not treated with venetoclax. It is also the only combination of these treatments that did not cause visible coral bleaching. Ctrl stands for control treatment, ven for venetoclax, AT, and HT for ambient and high-temperature treatment, respectively. Each coral fragment was live imaged three times (from different perspectives) in each timepoint and 10 different areas per picture were analyzed for green and red signal strength. Grey shadows represent a 95% confidence interval.
Figure S7