As expected, corals treated with colchicine showed approximately 4x increased activity of caspase-3 but not APs (Figure 5A). Conversely, corals treated with rapamycin increased the activity of APs (~ 1.5x) but not of caspase-3. This control experiment was important to i) assess the kits work for coral samples and ii) show the rate of enzymatic activity upregulation in corals that activate each programmed cell death pathway.