Differential ceRNA Expression and Interaction Analysis in Coronary
Background: Previous studies had shown that mRNA, miRNA and lncRNA were
associated with cardiovascular diseases. The study was aimed to explore
the differential expressions of mRNA, lncRNA and miRNA between coronary
artery disease (CAD)and healthy control, and their interaction in CAD.
Methods: We investigated the differential expression of ceRNA between
CAD and healthy control through data collected from Gene Expression
Omnibus (GEO) microarrays. Furthermore, we investigated the biological
function of these differential expressions of ceRNAs by Gene Ontology
(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.
Protein-protein interaction (PPI) network was created to identify the
hub genes. Biosystems and literature search were performed for signaling
pathways and their function of the included differential expression
ceRNAs. Results: A total of 456 miRNA expression profiles, 16,325 mRNA
expression profiles, and 2,869 lncRNA expression profiles were obtained.
Eleven Go and KEGG pathways (count ≥9), top 15 of PPI network node
connectivity rank, and top 15 of ceRNA network node degree centrality
rank were achieved at the statistical significance level
(P<0.05). We further identified that several differential
expressions of ceRNAs and their signaling pathways were associated with
CAD through biosystems and literature search. Conclusions: Based on
eleven Go and KEGG pathways, top 15 of PPI network node connectivity
rank, and top 15 of ceRNA network node degree centrality rank in CAD
population, our findings would contribute to further exploration for the
molecular mechanism of CAD.