Fig. 3 HSG4112 enhances energy expenditure in HFD-induced obese mice. For assessing in vivo energy expenditure, HFD-induced obese mice that were fed HSG4112 (0.5% feed mixture) for 4 weeks were placed in an Oxymax/CLAMS device (n = 4). c Oxygen consumption rate, d carbon dioxide production rate, e respiratory exchange ratio, and f energy expenditure were measured and normalized to body weight. g Locomotor activity was measured by number of beam breaks per hour. Data represent mean ± SEM. Two-way ANOVA with Sidak’s multiple comparison test was performed; *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle group. Grey area represents dark hours, starting at 8 p.m.