Histological analysis
Paraformaldehyde-fixed periepididymal fat and liver were paraffin-embedded, sectioned, and stained with Mayer’s hematoxylin-eosin (Sigma-Aldrich Co., St. Lousi, MO, USA). The NAFLD activity score and fibrosis staging system was applied to liver sections for scoring of steatosis, lobular inflammation, hepatocyte ballooning, and fibrosis as outlined by Kleiner et al (Kleiner et al. , 2005). Individual adipocyte size was calculated with ImageJ software (Schneider, Rasband and Eliceiri, 2012). All histological assessments were performed by a pathologist blind to treatment.