FIGURE 4. HSG4112 regulates metabolic gene expression,
influences AMPK protein level, and has no effect on UCP-1 level. (A-D)
Relative gene expression was measured using qRT-PCR (n = 4) on terminal
(A) gastrocnemius muscle, (B) liver, (C) hypothalamus, and (D)
interscapular adipose tissue (BAT) collected from the same mice from
Figure 2. Data represent mean ± SEM. *P < 0.05,
**P < 0.01 vs. Vehicle group,#P < 0.05,##P < 0.01 vs. Normal group;
one-way ANOVA with Tukey’s multiple comparison test. (E, F) HFD-mice
were treated for 11 days before sacrifice (n = 4 for AMPK groups, 3 for
UCP1 groups). (E) Western blot of phosphorylated hypothalamic AMPK ɑ1
and ɑ2 and their quantification. *P < 0.05, two-way
ANOVA with Sidak’s multiple comparison test; (F) Western blot of BAT
UCP1 and its quantification. n.s. = not significant; Student’s t-test.