Figure 2 YDZG inhibits NLRP3 expression. J774A.1 (A) or THP-1
(D) cells were pretreated with the indicated concentrations of YDZG for
6 h. NLRP3, caspase-1 and ASC expression was examined by Western
blotting(n=5). (B) J774A.1 cells were treated or untreated with YDZG at
10 μM for different times. NLRP3, caspase-1 and ASC expression was
examined by Western blotting (n=5). J774A.1 (C) and THP-1 (E) cells were
pretreated with 10 μM YDZG 1 h before being stimulated with LPS (1
μg·mL-1) for another 6 h, and were then treated with
ATP (5 mM) for 30 min before harvesting. NLRP3, caspase-1 and ASC
expression was examined by Western blotting (n=5). (F) Immunoblot
analysis of extracts from HEK293T cells transfected with NLRP3 plasmid
(n=5). (G) HEK293T cells were transfected with eGFP-NLRP3 plasmid for 24
h. Cells were pretreated with YDZG (20 μM) for 1 h before being
stimulated with LPS (1 μg·mL-1) for another 6 h, then
treated with ATP (5 mM) for 30 min (n=3). Cells were fixed and stained
with Hoechst 33342 (1 μM) for imaging (scale bar = 20 μm).