BALF preparation
BALF was harvested via injection and retraction of PBS-EDTA three times.
Approximately 0.8 mL BALF was acquired and centrifuged at 1000 × g for 5
min at 4 °C. The supernatant was immediately stored at -80 °C.
Data analysisAll data and statistical analyses comply with the recommendations for
experimental design and analysis in pharmacology. All results are
presented as the means ± SEM. For statistical analysis, the significance
of the intergroup differences was analyzed by one-way ANOVA with
Dunnett’s multiple comparisons test using GraphPad Prism 6.0 software. A
significant difference was defined as P < 0.05.