EXPERIMENTAL APPROACH
The in vitro anti-inflammatory effect and mechanism of YDZG were
investigated through ELISA, qRT-PCR, Western blotting, and
immunoprecipitation assay. ALI model was established by non-invasive
intratracheal instillation of LPS into lung of mice. The therapeutic
effect of YDZG on ALI was assessed by changes in histopathological and
pro-inflammatory markers. The underlying mechanisms were investigated by
Western blotting on lung tissue.