YDZG inhibits LPS-induced NF-κB activation
YDZG was found to suppress NF-κB activation in J774A.1 (Figure 3A),
THP-1 (Figure 3B), and RAW264.7 cells (Figure S2C), which is an
essential step for NLRP3 activation. LPS increased the release of
pro-inflammatory mediators such as NO (Figures S2A-B), TNF-α (Figure
S2G) and IL-6 (Figures S2F-H), which were reversed by YDZG. Furthermore,
immunofluorescence staining analysis revealed that YDZG inhibited the
LPS-induced translocation of NF-κB/p65 into the nucleus of macrophage
cells (Figure 3C, Figure S2E), which was consistent with the results of
the changes in cytoplasmic and nuclear NF-κB expression (Figure 3D;
Figure S2D). Overall, results suggested that YDZG blocked the NF-κB
pathway to suppress NLRP3 expression.