Histology
Lung tissue was harvested 24 h after LPS treatment. Lung tissue was fixed with 4% paraformaldehyde for 48 h, then dehydrated, embedded in paraffin, and sliced. The slices were stained with hematoxylin and eosin (H&E) and were viewed under a light microscope. NLRP3 expression was examined by immunohistochemistry using a rabbit anti-mouse NLRP3 antibody.