YDZG suppresses mitochondrial reactive oxygen species (ROS)
generation.
Reactive oxygen species (ROS) plays an important role in NLRP3
inflammasome assembly. Nuclear factor E2-related factor 2 (Nrf2) is a
basic leucine zipper redox-sensitive transcription factor known for its
antioxidant and anti-inflammatory properties (Zhang et al., 2013).
Previous studies indicated that mitochondrial ROS activates NLRP3
inflammasome activation through the Nrf2 pathway(Hu et al., 2018; Zhao
et al., 2014). In the present study, we investigated whether YDZG
inhibited ROS generation to block NLRP3. As shown in Figure 4A, B, and
E, YDZG sharply inhibited LPS-induced ROS generation. Using the Mito
Sox, a mitochondrial ROS fluorescence detector, we found that YDZG
inhibited mitochondrial ROS generation (Figure 4C). In addition, our
results indicated that YDZG promoted Nrf2 translocation from the
cytoplasm into the nucleus (Figure 4D-F), suggesting that YDZG inhibited
mitochondrial ROS generation via the Nrf2 pathway.