Figure 8 YDZG inhibits NLRP3 inflammasome activation in lung tissue. Mice were treated with LPS and YDZG as described in the Materials and Methods section. IL-1β in BALF (A), serum (B) and lung tissue (C) were determined using ELISA kits. (D) The expression of NLRP3 and caspase-1 p20 proteins was determined by Western blotting (n=5). (E) Immunohistochemical staining for NLRP3 in the lung tissues of mice challenged with LPS. Immunoreactivity is shown in brown (scale bar = 100 μm). Data are reported as mean ± SEM; n = 8; *P < 0.05 compared with the LPS alone group.