Reagent
YDZG (>98%) was isolated in our laboratory and its purity
was determined by HPLC. LPS (Escherichia coli , serotype 0111: B4)
was purchased from Sigma-Aldrich (St. Louis, MO, USA). DMEM and FBS were
purchased from Life Technologies/Gibco Laboratories (Grand Island, NY,
USA). ELISA kits for IL-1β, IL-6 and TNF-α were purchased from
Neobioscience (Shenzhen, China). Antibodies against p65, p-p65,
p-IKK-α/β, IKK-α, IKK-β, p-IκB-α, IκB-α, NLRP3, IL-1β, cleaved-IL-1β,
caspase-1, cleaved-caspase-1, and GAPDH were purchased from Cell
Signaling Technologies (Beverly, MA, USA). Antibodies against TRIM31
were purchased from Thermo Fisher Scientific (Grand Island, NY, USA).
Oligonucleotide primers for Nlrp3, IL-1β and GAPDH were synthesized by
Invitrogen (Nanning, China).
4-Amino-5-methylamino-20,70-difluorofluorescein diacetate (DAF-FM), a
protein A/G magnetic bead kit, RT-PCR, and quantitative PCR (qPCR) kits,
were purchased from Life Technologies/Thermo Fisher Scientific (Grand
Island, NY, USA). Plasmids of pcDNA3-N-Flag-NLRP3 and pEGFP-C2-NLRP3
were purchased from Addgene (Beijing, China).