EXPERIMENTAL APPROACH
The in vitro anti-inflammatory effect and mechanism of YDZG were investigated through ELISA, qRT-PCR, Western blotting, and immunoprecipitation assay. ALI model was established by non-invasive intratracheal instillation of LPS into lung of mice. The therapeutic effect of YDZG on ALI was assessed by changes in histopathological and pro-inflammatory markers. The underlying mechanisms were investigated by Western blotting on lung tissue.