YDZG suppresses mitochondrial reactive oxygen species (ROS) generation.
Reactive oxygen species (ROS) plays an important role in NLRP3 inflammasome assembly. Nuclear factor E2-related factor 2 (Nrf2) is a basic leucine zipper redox-sensitive transcription factor known for its antioxidant and anti-inflammatory properties (Zhang et al., 2013). Previous studies indicated that mitochondrial ROS activates NLRP3 inflammasome activation through the Nrf2 pathway(Hu et al., 2018; Zhao et al., 2014). In the present study, we investigated whether YDZG inhibited ROS generation to block NLRP3. As shown in Figure 4A, B, and E, YDZG sharply inhibited LPS-induced ROS generation. Using the Mito Sox, a mitochondrial ROS fluorescence detector, we found that YDZG inhibited mitochondrial ROS generation (Figure 4C). In addition, our results indicated that YDZG promoted Nrf2 translocation from the cytoplasm into the nucleus (Figure 4D-F), suggesting that YDZG inhibited mitochondrial ROS generation via the Nrf2 pathway.