Figure 5 YDZG enhances TRIM31 expression. J774A.1 (A) and THP-1 (C) cells were pretreated with the indicated concentrations of YDZG for 6 h. TRIM31 expression was examined by Western blotting(n=5). J774A.1 (B) or THP-1 (D) cells were pretreated with 10 μM YDZG or 1 h before being stimulated with LPS (1 μg·mL-1) for another 6 h, and were then treated with ATP (5 mM) for 30 min before harvesting. TRIM31 expression was examined by Western blotting (n=5). (E) THP-1 cells were pretreated with YDZG at 40 μM for 1 h before being stimulated with LPS (1 μg·mL-1) for another 6 h, and were then treated with ATP (5 mM) for 30 min before harvesting. Proteins were isolated and immunoprecipitated with an antibody against NLRP3 (n=5).