YDZG reduces LPS-induced infiltration of inflammatory cells and cytokine levels.
LPS-treated mice had more leukocyte (Figure 7A) and neutrophil (Figure 7B) counts in blood than that of the control. However, LPS- and YDZG-treated mice had significantly fewer leukocytes and neutrophils than mice treated with LPS alone. Moreover, LPS-challenged animals showed significantly greater MPO activity in lung tissue (a marker for neutrophils) than the control. Meanwhile, YDZG pretreated-mice had less MPO activity than those treated with LPS alone (Figure 7C). In the LPS-induced ALI model, YDZG decreased the LPS-induced inflammatory cytokines TNF-α (Figure 7D) and IL-6 (Figure 7E) in BALF. Similar results were found in serum (Figures 7F- G) and lung tissue samples (Figures 6H- I), respectively. Thus, YDZG reduced the infiltration of inflammatory cells and cytokine levels to prevent ALI.