YDZG ameliorates LPS-induced acute lung injury in mice.
A lethal dose of LPS was intratracheally injected into lung tissue of mice with non-invasive assay, and their survival rate were recorded. In the LPS treated group, all mice died within 96 h after LPS injection, however, mice pretreated with YDZG (5 mg·kg-1) had a significantly greater survival rate (Figure 6A). In addition, results of respiratory mechanic analysis indicated that LPS increased lung functional indexes like the resistance of lung (RL) and resistance of expiration (Re), and decreased respiratory lung compliance (Cdyn), of which the effects of RL (Figure 6B), Re (Figure 6C), and Cdyn (Figure 6D) were significantly were reversed by YDZG pretreatment. Furthermore histological analysis indicated that LPS triggered the infiltration of antibacterial cells, thickened the alveolar wall, and destroyed the normal lung tissue structure; YDZG treated groups prevented LPS-induced acute lung injury (Figure 6E).