Figure. 1. Ziyuglycoside II inhibits colorectal cancer cells
proliferation both in vitro and in vivo
(A ). Chemical structure of Ziyu II. MTT assay of colorectal
cancer lines treated with the indicated concentrations of Ziyu II for
24h (B ). EdU incorporation (C ), LDH release assay in
cells treated as in (D ). Colony formation assay of DLD-1 and
HCT116 cells treated with the indicated concentrations of Ziyu II for
2wks. Representative images (E ) and quantification of colonies
(F ) were shown. G–J, NOD-SCID mice were inoculated with DLD-1
cells and treated with Ziyu II or vehicle. Tumor volumes were measured
at indicated time points (I ). Photograph of isolated tumors
derived from control or Ziyu II-treated mice (G ). Tumor weights
at time of sacrifice (H ). Ki67 expression in tumor xenografts
was examined by IHC(J ), and relative immunohistochemical scores
were shown (K ) Scale bar, 50 μm. All data are means±SD. **,p < 0.01; ***, p < 0.001.