Figure 2. Ziyuglycoside II induces apoptosis in colorectal cancer cells in vitro
DLD-1 and HCT116 cells were subjected to Ziyu II for 24 h, and flow cytometric analysis of apoptosis (A ). (B ) MTT assay of DLD-1 and HCT116 cells treated with the presence or absence of Ziyu II, and in combination with or without ZVAD for 24h. Colony formation assay of DLD-1 and HCT116 cells treated with the presence or absence of Ziyu II, and in combination with or without ZVAD for 2wks. Representative images (C ) and quantification of colonies (D ) were shown. (E ) Flow cytometric analysis of ROS in DLD-1 and HCT116 cells treated with the indicated concentration of Ziyu II for 24h. (F ). MTT assay of DLD-1 and HCT116 cells treated with NAC for 24h. All data are means±SD. *, p < 0.05; **,p < 0.01; ***, p < 0.001.