Supplementary figure4. Inhibition of autophagy represses the
antiproliferative effect of Ziyu II in colorectal cancer cells. Colony
formation assay of DLD-1 and HCT116 cells were treated with or without
3-MA in the presence or absence of Ziyu II. Representative images
(A ) and quantification of colonies (C ) were shown. MTT
assay (B ) of ells were treated with or without 3-MA in the
presence or absence of Ziyu II. LDH release assay in cells treated as in
(B ). (F ). Cells were transfected with an empty vector
(pECE) or with a constitutively active CA-Akt for 48 hours, and then
treated with Ziyu II. the cytotoxicity was detected by the release of
LDH. All data are means±SD. *, p < 0.05; **, p< 0.01.