Supplementary figure 2. Ziyuglycoside II induces apoptosis in colorectal cancer cells both in vitro and invivo Immunoblot analysis of Cleaved-PARP in cells treated with the indicated concentrations of Ziyu II for 24 hours (A). Immunoblot analysis of Cleaved-PARP in cells treated with ZVAD in the presence or absence of Ziyu II (20 uM) for 24h (B). Immunohistochemical staining of cleaved-caspase 3 (CC3) (C) in tumors from vehicle or Ziyu II-treated mice bearing DLD-1 subcutaneous tumor xenografts. Relative immunohistochemical scores were shown (D). Scale bar, 50 μm. ***, p< 0.001.