Lactate dehydrogenase release assay
Lactate dehydrogenase (LDH) test kit (Beyotime Biotechnology, Nanjing,
China) was used to assess the cytotoxicity of Ziyu II. Cells were
cultured in 96-well plates (6x103 cells/well). After
treatment with various concentrations of Ziyu II for 24h, cell culture
supernatant was transferred to the new 96-well plate for LDH analysis.