Supplementary figure 3. Interfering with ATG5 can inhibit the formation of LC3 puncta in CRC (A ). Cells were transfected with an empty vector or with siAtg5 for 48 hours, and then cells were treated with Ziyu II (20 uM) for another 24 hours. Atg5 was determined by immunoblotting and (B , D ) the formation of endogenous LC3 puncta was assessed in cells treated as in A and (C , E ) total number of endogenous LC3 puncta per cell. Scale bars: 10 μm. All data are means±SD. ***, p < 0.001.