Immunohistochemical analysis
Immunohistochemical analysis was performed as described previously
[44]. All samples were observed under a Leica DM 2000 microscope.
The percentage of positive cell area (a) was multiplied by the intensity
of immunostaining (B: 0, negative; 1, weak positive; 2, positive; 3,
strong positive). The final score of each slide is calculated as a × B.
The final score for each slide was calculated as A×B.