Figure 6 . Inhibition of autophagy represses the
antiproliferative effect of Ziyu II in colorectal cancer cells.
(A ). MTT assay of DLD-1 and HCT116 cells were treated with or
without CQ in the presence or absence of Ziyu II. Representative images
(B ) and quantification of colonies (C ) were shown.
(D ) LDH release assay in cells treated as in (A ). (F)
MTT assay of DLD-1 and HCT116 cells were transfected with an empty
vector (pECE) or with a constitutively active CA-Akt for 48h, and then
treated with Ziyu II. Colony formation assay of DLD-1 and HCT116 cells
were transfected with an empty vector (pECE) or with a constitutively
active CA-Akt for 48h, and then treated with Ziyu II. Representative
images (G ) and quantification of colonies (H ) were
shown. All data are means±SD. *, p < 0.05; **, p< 0.01.
Supplementary Materials