Figure. 1. Ziyuglycoside II inhibits colorectal cancer cells proliferation both in vitro and in vivo
(A ). Chemical structure of Ziyu II. MTT assay of colorectal cancer lines treated with the indicated concentrations of Ziyu II for 24h (B ). EdU incorporation (C ), LDH release assay in cells treated as in (D ). Colony formation assay of DLD-1 and HCT116 cells treated with the indicated concentrations of Ziyu II for 2wks. Representative images (E ) and quantification of colonies (F ) were shown. G–J, NOD-SCID mice were inoculated with DLD-1 cells and treated with Ziyu II or vehicle. Tumor volumes were measured at indicated time points (I ). Photograph of isolated tumors derived from control or Ziyu II-treated mice (G ). Tumor weights at time of sacrifice (H ). Ki67 expression in tumor xenografts was examined by IHC(J ), and relative immunohistochemical scores were shown (K ) Scale bar, 50 μm. All data are means±SD. **,p < 0.01; ***, p < 0.001.