Figure 6 . Inhibition of autophagy represses the antiproliferative effect of Ziyu II in colorectal cancer cells.
(A ). MTT assay of DLD-1 and HCT116 cells were treated with or without CQ in the presence or absence of Ziyu II. Representative images (B ) and quantification of colonies (C ) were shown. (D ) LDH release assay in cells treated as in (A ). (F) MTT assay of DLD-1 and HCT116 cells were transfected with an empty vector (pECE) or with a constitutively active CA-Akt for 48h, and then treated with Ziyu II. Colony formation assay of DLD-1 and HCT116 cells were transfected with an empty vector (pECE) or with a constitutively active CA-Akt for 48h, and then treated with Ziyu II. Representative images (G ) and quantification of colonies (H ) were shown. All data are means±SD. *, p < 0.05; **, p< 0.01.
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