Plasmids
The human AKT (NM_001014431.1) coding region with GFP-Tag was cloned using PCR and was ligated into the pEGFP-N1 vector. The origin PCR primers for AKT cds sequence were as follows: Sense primer: 5’ CCG GAA TTC ATG AGC GAC GTG GCT ATT 3’; Anti-sense primer: 5’CGC GGA TCC CCG GCC GTG CCG CTG GCC GA 3’. The constitutively active form of Akt (CA-Akt, myrAkt delta4-129) was purchased from Addgene. Flag-Ubiquitin was a kind gift from Professor Changan Jiang (Sichuan University, China). The plasmids were introduced into cells using Lipofectamine 3000 according to the manufacturer’s protocol.