Cell culture
Human colorectal cancer cell lines (HCT116, HT29, DLD-1, SW620 and
SW480) and the human normal colonic epithelial cell line NCM460 were
obtained from the ATCC (Manassas, VA, USA) and cultured in DMEM (Thermo
Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine
serum (Thermo Fisher Scientific), 100 U/ml penicillin (Millipore Sigma,
Burlington, MA, USA), and 100 U/ml streptomycin (Millipore Sigma) at
37°C in 5% CO2.