2.3 Solvent extraction of Qingjinju seeds
Qingjinju seeds were ground
(model
YC-04B, Shanghai Jinben Machinery Co., Ltd., Shanghai, China) for 30 s
and mixed with hexane in a ratio 1:7 (w/v). The mixture was transferred
to a closed glass reaction kettle and stirred for 2 h at
55 °C in a water bath (W-O, Shanghai
Shengshun Biotechnology Co., Ltd.,
Shanghai, China). The solution was decanted and the extraction was
repeated three times. The solutions were then evaporated under reduced
pressure at 60 °C and stored at 3 °C in a refrigerator.