2.1 Materials
Qingjinju (C. microcarpa (Bunge), Rutaceae) seeds were gifted by
Hainan Qingjinju Food Technology Co., Ltd. (Hainan, China) during the
2017-2018 harvest season. Approximately 10 kg of the seeds were washed,
drained, and oven-dried (DAIHAN
SOF-155, DAIHAN Scientific Co., Ltd., Wonju-si, Korea) at
50 °C for 5-8 h. The seed moisture
content was monitored and maintained at 4.0%. Seeds were stored in a
refrigerator at 3 °C prior to use.
All chemicals used in the study were of analytical and/or
chromatographic grade and were purchased from Shanghai Yuanye
Bio-Technology Co.,
Ltd. (Shanghai, China). Standards
used for chromatographic analyses were purchased from ANPEL Laboratory
Technologies (Shanghai) Inc. (Shanghai, China).