2.1 Fruit harvest and sample preparation
Cannabis sativa L. seeds from two different varieties,Futura 75 and Carmagnola , were picked in Ancona area in Italy approximately 43° 30’ 34286” N, 13°15’ 33052” E. Fruits were harvested at three different harvesting stages during August and September 2015. At first harvest, Futura 75 were collected on August 26th (F1) and Carmagnola on September 3rd (C1); at the second harvest, Futura 75 were collected on September 3rd (F2) and Carmagnolaon September 15th (C2); and at third harvest,Futura 75 were collected on September 15th (F3) and Carmagnola on September 23rd (C3). At each sampling time 20 g of seeds were picked; all of them were grounded before analyses.