2.2 Oil extraction and moisture content determination
The lipid content was obtained by grounding the samples ofCannabis sativa L. seeds (5 g) and the oil was extracted withn- hexane in a Soxhlet apparatus according to ISO method 659:1998.
The remaining solvent was removed under vacuum and the oil was taken up
with n -hexane/isopropanol (4:1 v/v) solution and stored at−18 °C
until use. Each extraction was carried out two times for each cultivar.
Moisture content (%) was evaluated on hemp seeds samples in an oven at
105 °C until constant weight was receached. For each samples, 3
replicates of 3 g weighted were dried (AOAC, 1995)..