2.1. Materials
Expanded
rice bran were purchased from Fushi Rice Industry Ltd,
Kiamusze,
Heilongjiang Province, China.
In
order to prevent rancidity, the raw materials were stored in the
refrigerator at -20
℃.
Celluclast 1.5L, Pectinex Ultra SP-L, Viscozyme L and Alcalase 2.4L were
purchased from
Novozymes
Bagsvaerd
(Denmark).
Hemicellulase and the standard of tocopherols, tocotrienols, sterols and
squalene were obtained
from
Sigma-Aldrich Co. (Steinheim, Germany).
Fatty
acid methyl esters (FAMEs) standards were obtained from the AccuStandard
(New Haven, CT,
USA).
Additionally, all other chemicals were of analytical grade.