2.4. Physicochemical properties
AOCS Standard Methods (1997) were used for the determination of the acid
value (method Cd 3d-63), iodine value (method Cd 1-25), saponification
value
(method
Cd 3-25) and peroxide value (method Cd 8-53) of AEEO and SEO.
The wax and phospholipid content of
RBO
were measured according to the method reported by Pandey et al. (2018).
Specifically, wax content in RBO was measured as acetone insoluble
fraction and phospholipid content of RBO was measured by the
Vanadomolybdate method.
The
colour parameters of oil samples were measured using chroma meter CR-400
(Konica Minolta Sensing, Inc., Osaka, Japan). Before using the chroma
meter, it was necessary to correct. A certain amount of oil samples were
taken in the cuvette, then used the chroma meter to irradiation. The
data of L-value, a-value, b-value and colour difference (△E) were
recorded.