2.4. Physicochemical properties
AOCS Standard Methods (1997) were used for the determination of the acid value (method Cd 3d-63), iodine value (method Cd 1-25), saponification value (method Cd 3-25) and peroxide value (method Cd 8-53) of AEEO and SEO.
The wax and phospholipid content of RBO were measured according to the method reported by Pandey et al. (2018). Specifically, wax content in RBO was measured as acetone insoluble fraction and phospholipid content of RBO was measured by the Vanadomolybdate method.
The colour parameters of oil samples were measured using chroma meter CR-400 (Konica Minolta Sensing, Inc., Osaka, Japan). Before using the chroma meter, it was necessary to correct. A certain amount of oil samples were taken in the cuvette, then used the chroma meter to irradiation. The data of L-value, a-value, b-value and colour difference (△E) were recorded.