Inactivation of LPS by recIAP at neutral pH
recIAP exhibited a significant effect on LPS inactivation at neutral
pH7.5 (Table 1), indicating its LPS-inactivating effect in human blood
and tissues. High-dose recIAP (10 U ml-1) reduced the
activity of LPS (5ng ml-1) up to 50% within 3 hours
of incubation at 37°C (Figure 1). In addition, incubation of higher-dose
recIAP(50 U ml-1) and higher-dose LPS(1μg
ml-1) at 37℃ for one hour reduced LPS activity by
approximately 60% at pH 7.0 and 7.5, while LPS activity decreased by
76.85% at pH 8.0.