bIAP, as well as recIAP, inhibit the secretion of TNF - α and IL-6
To study the effect of extracted bovine intestinal alkaline phosphatase (bIAP) on leukocyte secretion of TNF-α and IL-6, bIAP was subjected to the same cell culture models as recIAP. The results showed that bIAP (5U ml-1) significantly inhibit human leukocytic secretion of TNF-α and IL-6 in the presence of LPS (0.5ng ml-1) (Figure 4 black bar). bIAP also exhibited a significant inhibitory effect on TNF-α and IL-6 in the absence of LPS (Figure 4 white bar). The inhibitory effect of bIAP and recIAP on TNF-α and IL-6 secretion which has been demonstrated in our experiment reflects the in vivo function of IAP expressed on the surface of intestinal mucosa to act in an anti-inflammatory capacity against fecal endotoxin content released by E. coli (Poelstra, Bakker, Klok , Hardonk, Meijer, 1997; Kaliannan, 2013; Narisawa, 2003; Peters, 2016a; Lukas,2013).