Detection of intestinal alkaline phosphatase activity
Alkaline phosphatase activity was measured using a phenyl disodium phosphate colorimetry kit (TE0007, Beijing Regen Biotechnology Co., Ltd.). Phenyl disodium phosphate is hydrolyzed by alkaline phosphatase under alkaline conditions to form free phenol and phosphoric acid. Under basic conditions, phenol is combined with amino antipyrine and oxidized to form red quinone structures with different shades. The absorbance was measured at 510 nm and the alkaline phosphatase activity level was calculated by colorimetric analysis. An active unit (U L-1) is defined as 100 milliliters of sample at 37°C for 15 minutes of incubation with benzene disodium phosphate in order to produce 1 milligram of phenol.