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High-level EPA production from Phaeodactylum tricornutum
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  • Yi Cui,
  • Skye Thomas-Hall,
  • Elvis Chua,
  • Peer Schenk
Yi Cui
University of Queensland
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Skye Thomas-Hall
University of Queensland
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Elvis Chua
University of Queensland
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Peer Schenk
University of Queensland
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Abstract

Phaeodactylum tricornutum is a lipid-rich marine diatom that contains a high level of omega-3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA). In an effort to reduce costs for large-scale cultivation of this microalga, we first established a modified BBM medium (0.3 x strength BBM with 90% reduced phosphate content) to replace the traditional F/2 medium. P. tricornutum could grow in extremely low phosphate concentrations (25 µM), without compromising the EPA content. In the presence of sea salts, silicate addition was not necessary for high rate growth, high EPA content or lipid accumulation in this species. Using urea as the sole nitrogen source tended to increase EPA contents (by 24.7%) while not affecting growth performance. The use of sea salts, rather than just sodium chloride led to significantly improved biomass yields (20% increase) and EPA contents (46-52% increase), most likely because it supplied sufficient essential elements such as magnesium. A salinity level of 35 ppt led to significantly higher biomass yields compared to 20 ppt, but salinity had no significant influence on EPA content that reached high average levels of 51.8% of total fatty acids during exponential growth phase at 20 ppt in modified BBM medium with sea salts.