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Chromosome-level de novo genome assembly of Sarcophaga peregrina provides insights into the evolutionary adaptation of flesh flies
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  • Lipin Ren,
  • Yanjie Shang,
  • Li Yang,
  • Shiwen Wang,
  • Xiang Wang,
  • Shan Chen,
  • Zhigui Bao,
  • Dong An,
  • Fanming Meng,
  • Jifeng Cai,
  • Yadong Guo
Yanjie Shang
Central South University
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Li Yang
Central South University
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Shiwen Wang
Xin Jiang Medical University
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Xiang Wang
Dalian Medical University
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Shan Chen
East China Normal University
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Zhigui Bao
OE biotech Co Ltd
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Dong An
OE biotech Co Ltd
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Fanming Meng
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Jifeng Cai
Central South University
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Yadong Guo
Central South University
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Peer review status:IN REVISION

11 Mar 2020Submitted to Molecular Ecology Resources
15 Mar 2020Reviewer(s) Assigned
11 May 2020Review(s) Completed, Editorial Evaluation Pending
27 May 2020Editorial Decision: Revise Minor
26 Jun 2020Review(s) Completed, Editorial Evaluation Pending
26 Jun 20201st Revision Received

Abstract

Sarcophaga peregrina is usually considered to be of great ecological, medical and forensic significance, and has the biological characteristics such as the ovoviviparous reproductive pattern and adaptation to feed on carrion. However, the underlying mechanisms still remain unsolved by lack of high-quality genome. Here we present de novo‚Äďassembled genome at chromosome-scale for S. peregrina. The final assembled genome was 560.31 Mb with contig N50 of 3.84 Mb. Hi-C scaffolding reliably anchored six pseudochromosomes, accounting for 97.76% of the assembled genome. Moreover, 45.70% of repeat elements were identified in the genome. A total of 14,476 protein-coding genes were functionally annotated, accounting for 92.14% of all predicted genes. Phylogenetic analysis indicated that S. peregrina and S. bullata diverged ~7.14 Mya. Comparative genomic analysis revealed expanded and positively selected genes related to biological features that aid in clarifying its ovoviviparous reproduction and necrophagous habit, such as horionic membrane formation and Dorso-ventral axis formation, lipid metabolism, and olfactory receptor activity. This study provides a valuable genomic resource of S. peregrina, and sheds insight into further revealing the underlying molecular mechanisms of adaptive evolution.