3.2 Active phase INS patients Th2 cells were over-activation and the expression of associated factors were increased.
The number of Tregs from patients’ whole blood was quantified by flow cytometry (Fig. 2). The proportion of peripheral Th2 cells in initial atopic group (5.60±1.21 vs. 3.29±1.02,P<0.05) and the initial non-atopic group (4.23±0.92 vs. 3.29±1.02,P<0.05) were significantly increased than that of healthy subjects, there was no significant difference between remission atopy group and remission non-atopic group (3.60±0.85 vs. 3.33±0.95,P > 0.05 ) ; Compared with the initial atopic group, the other groups were significantly decreased; Compared with the initial non-atopic group, the initial atopic group was significantly higher, the control and the remission non-atopic group were significantly reduced, and there was no significant difference in the remission atopic group (Fig. 2A, B). We detected plasma levels of IL-4 and IL-13 by CBA (Fig. 2C, D) and found that plasma IL-4 and IL-13 concentrations were significantly increased in initial atopic group (IL-4: 66.66±43.85 vs. 23.29±13.91,P < 0.05; IL-13: 65.15±16.50 vs. 15.66±8.16,P < 0.05) and the initial non-atopic group (IL-4: 37.04±17.39 vs. 23.29±13.91, P < 0.05; IL-13: 33.15±14.09 vs. 15.66±8.16, P < 0.05), there was no significant difference between remission atopy group and remission non-atopic group (22.09±10.32 vs. 16.47±11.69, P > 0.05 ); Compared with the initial atopic group, the other groups were significantly decreased; Compared with the initial non-atopic group, the initial atopic group was significantly higher, the control and the remission non-atopic group were significantly reduced, and there was no significant difference in the remission atopic group.