Fig.4. and Fig.5. MiR-24 and miR-27 negative regulated the expressions of Th2 cells. Relative expression levels of miRNAs or IL-4 were determined by real-time PCR using U6 or GAPDH as an endogenous reference gene. Normalized values are derived from the ratios of target miR/U6 or gene/GAPDH mRNA expression, shown as mean ± SD. Six children with active non-atopic INS and six control subjects were enrolled in this study. Data are shown as mean ± SD. *P < 0.05. **P > 0.05. Ctrl, healthy control; INS, idiopathic nephrotic syndrome; Ctrl-m, miRNA negative control; miR-24-m/miR-27-m, miR-24/miR-27 mimic; Ctrl-i, miRNA inhibitor negative control; miR-24-i/miR-27-i, miR-24/miR-27 inhibitor.