Figure 1 : Species detection using eDNA deposited on vegetation surfaces is contingent on several factors. First, an appropriate eDNA state must be selected for the question being addressed, as well as the correct filter pore size to effectively collect said state. Second, the transport of eDNA must be taken into consideration when preparing a sampling protocol. Rainfall will be the predominant influence facilitating the transport of eDNA from the vegetation surface onto another substrate or, more likely, into the soil below. Third, the fate of eDNA will affect detection, as abiotic factors such as solar radiation (UV) will result in cells and organelles being destroyed (i.e. cell lysis), followed by fragmentation of extracellular eDNA.