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Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize
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  • Dyoni Oliveira,
  • Thatiane Mota,
  • Fábio Salatta,
  • Renata Sinzker,
  • Radka Končitíková,
  • David Kopečný,
  • Rachael Simister,
  • Mariana Silva,
  • Geert Goeminne,
  • Kris Morreel,
  • Jorge Rencoret,
  • Ana Gutiérrez,
  • Theodora Tryfona,
  • Rogério Marchiosi,
  • Paul Dupree,
  • José del Río,
  • Wout Boerjan,
  • Simon McQueen-Mason,
  • Leonardo Gomez,
  • Osvaldo Ferrarese-Filho,
  • Wanderley dos Santos
Dyoni Oliveira
State University of Maringá,
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Thatiane Mota
State University of Maringá
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Fábio Salatta
State University of Maringá
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Renata Sinzker
State University of Maringá
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Radka Končitíková
Palacký University
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David Kopečný
Palacký University
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Rachael Simister
University of York
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Mariana Silva
University of York
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Geert Goeminne
Ghent University
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Kris Morreel
Ghent University
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Jorge Rencoret
Instituto de Recursos Naturales y Agrobiología de Sevilla
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Ana Gutiérrez
Instituto de Recursos Naturales y Agrobiología de Sevilla
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Theodora Tryfona
University of Cambridge
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Rogério Marchiosi
State University of Maringá
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Paul Dupree
University of Cambridge
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José del Río
Instituto de Recursos Naturales y Agrobiología de Sevilla
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Wout Boerjan
Ghent University
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Simon McQueen-Mason
University of York
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Leonardo Gomez
University of York
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Osvaldo Ferrarese-Filho
State University of Maringá
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Wanderley dos Santos
State University of Maringá
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Abstract

Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored. Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants. Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin. The extraction and analysis of arabinoxylans by size-exclusion chromatography, two-dimensional NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots, with no changes in xylose/arabinose ratios. Saponification and mild acid hydrolysis followed by RP-HPLC analysis revealed that salt stress also reduced the feruloylation of arabinoxylans. Determination of lignin content and composition by nitrobenzene oxidation and two-dimensional NMR confirmed the increased incorporation of syringyl units in lignin polymer. Our data also revealed the induction of the expression of genes and enzymes enrolled in phenylpropanoid biosynthesis under salinity. The UPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate. In conclusion, we present a model for explaining cell wall remodeling in response to salinity.

Peer review status:UNDER REVIEW

20 Mar 2020Submitted to Plant, Cell & Environment
23 Mar 2020Submission Checks Completed
23 Mar 2020Assigned to Editor
29 Mar 2020Reviewer(s) Assigned
13 Apr 2020Review(s) Completed, Editorial Evaluation Pending
27 Apr 20201st Revision Received
28 Apr 2020Submission Checks Completed
28 Apr 2020Assigned to Editor
07 May 2020Reviewer(s) Assigned
14 May 2020Review(s) Completed, Editorial Evaluation Pending