Phylogenetic tree reconstruction and domain analysis of
PtrWRKY75
The amino acid sequences of P. trichocarpa and Arabidopsis
WRKY genes were obtained from the PlantTFDB
(http://planttfdb.cbi.pku.edu.cn/index.php) and TAIR 9.0
(http://www.Arabidopsis.org/index.jsp) databases. The sequences ofWRKY genes from other species were used as queries for BLAST
searches against the P. trichocarpa genome database in the
catalogue of WRKY proteins in NCBI. The sequence accession numbers are
shown in Table S2-S3. A phylogenetic tree for WRKY genes was
constructed using the neighbor-joining method with MEGA5. DNAMAN 5
(Lynnon Biosoft Inc., San Ramon, CA, USA) was used to analyze the
deduced amino acid sequences of PtrWRKY75 and other WRKY family members
in Arabidopsis .