Phylogenetic tree reconstruction and domain analysis of PtrWRKY75
The amino acid sequences of P. trichocarpa and Arabidopsis WRKY genes were obtained from the PlantTFDB (http://planttfdb.cbi.pku.edu.cn/index.php) and TAIR 9.0 (http://www.Arabidopsis.org/index.jsp) databases. The sequences ofWRKY genes from other species were used as queries for BLAST searches against the P. trichocarpa genome database in the catalogue of WRKY proteins in NCBI. The sequence accession numbers are shown in Table S2-S3. A phylogenetic tree for WRKY genes was constructed using the neighbor-joining method with MEGA5. DNAMAN 5 (Lynnon Biosoft Inc., San Ramon, CA, USA) was used to analyze the deduced amino acid sequences of PtrWRKY75 and other WRKY family members in Arabidopsis .