Short-term and long-term drought experiments
Thirty WT and 60 oxPtrWRKY75 (30 oxPtrWRKY75-8 and 30oxPtrWRKY75-10 ) two-month-old plants were used as experimental
materials. For the short-term drought experiment, plants in pots
(150 cm width and 135 cm height) were
subjected to drought by withholding watering for 7 days. During this
period, net CO2 assimilation, G s,
and transpiration were measured daily. For the long-term drought
experiment, plants were grown in pots (150 cm width and 135 cm height)
with 70% soil RWC (no stress) or 20% soil RWC (severe water stress),
which was achieved by withholding water for 35 days (Wang et al. ,
2016). The soil RWC was measured daily. The height of each plant was
measured every 10 days. After 35 days, the photosynthesis rate, leaf
RWC, chlorophyll content, and plant aboveground biomass were measured.