PtrWRKY75 overexpression enhanced WUE by reducing
transpiration
Previous studies have suggested that ROS accumulation in leaves may
cause stomatal closure (Song et al. , 2014; Sierla et al. ,
2016), thereby reducing transpiration and improving the WUE of plants
(Yoo, C.Y et al ., 2009; Yoo, C.Y et al ., 2010). The
photosynthesis–light curve showed that photosynthesis was similar in
the OE lines and WT
(Figure
4A). The stomatal conductance (G s) was
significantly lower in the OE lines than in WT
(Figure 4B). The leaf transpiration
rate was about 30% lower in the OE lines than in WT
(Figure
4C). As a result, the instantaneous WUE (photosynthesis/transpiration)
values of the OE lines were significantly higher than those of WT
(Figure 4D). The vapor pressure
deficit (VPD) showed no significant difference between the OE lines and
WT, indicating that VPD was not a factor in the reduced transpiration
rate of the OE lines
(Figure
4E). In general, overexpression of PtrWRKY75 enhanced WUE by
reducing the transpiration rate.