Figure 2 PtrWRKY75 promotes salicylic acid (SA)-induced
stomatal closure via reactive oxygen species (ROS) accumulation.
(A) DAB staining to visualize ROS content in leaves of wild-type (WT)
and PtrWRKY75 -overexpressing (OE) lines in response to 0.5 mM SA
treatment for 0 h, 0.5 h, 1 h, 2 h, and 3 h. Scale bars = 2 cm. Data are
means ± SE (n = 6).
(B) SA-induced stomatal closure in leaves of WT and OE lines. Leaves
were detached and immersed in stomata-opening solution for 2 h under
light, then treated with 0.5 mM SA for 1 or 2 h. Scale bars = 10 μm.
Data are means ± SE (n = 60).
(C) Stomatal aperture observed at 0, 1, and 2 h of SA treatment by
scanning electron microscopy. Data are means ± SE (n = 60). Data
were analyzed using one-way analysis of variance followed by Duncan’s
multiple range test. Asterisks denote significant differences:
**P < 0.01.