Molecular verification
The cetyltrimethylammonium bromide (CTAB) method was used to extract
genomic DNA from non-transgenic plants (WT) and transgenic OE lines
(Arseneau et al. , 2017). We confirmed the transformation lines by
PCR using the combination of a forward primer for PtrWRKY75 and a
reverse primer for eGFP (Table S1). Quantitative real-time PCR was used
to determine the expression levels of PtrWRKY75 in the transgenic
lines and WT using the afore-mentioned procedure. The primers used were
identical to those used for the qRT-PCR assay listed in Table S1.