PtrWRKY75 promotes expression of SA-responsive genes
Our results indicated that PtrWRKY7 5 promotes SA biosynthesis by
binding to the promoter of PtrPAL1 , subsequently promoting ROS
accumulation (Figure 2). Consistent with these results, the expression
of PtrPR1 and PtrPR5 , two widely studied SA-responsive
genes (Blanco et al. , 2009), was significantly up-regulated in
OE plants under dehydration
conditions (Figure 7A, B). The results indicated that PtrWRKY75increased SA production by inducing PAL1 transcription, leading
to the expression of SA-responsive genes. Thus, our results show that
SA-induced ROS production, which leads to stomatal closure (Khokonet
al. , 2011;
Moriet al. , 2001), enhances drought tolerance in Populus .