Subcellular localization assay
For the subcellular localization assay,
Super:PtrWRKY75-eGFP
and Super:eGFP fusion proteins, which were transiently transfected into
tobacco leaves as previously described (Cui et al. , 2007), were
observed under a
confocal
laser scanning microscope (DMI6000 CS; Leica, Wetzlar, Germany).