Analysis of SA content
Two-month-old WT and OE lines were used as experimental materials. The
leaves of plants grown under well-watered conditions and dehydration for
4 h were collected and immediately frozen in liquid nitrogen. The leaves
were ground into powder in liquid nitrogen, and 50 mg powder was used
for quantitative free-SA measurements by high-performance liquid
chromatography–tandem mass spectrometry (Pan et al. , 2010).