5.
CONCLUSION
The strain Arthrobacter sp. ZJUTW exhibited high degradation
efficiency towards DBP. Based on the results of the genome sequencing
and the transcriptome, we found the key gene pehA and thepht gene cluster and pca gene cluster involved in DBP
degradation. The pehA and pht gene cluster are located in
plasmid pQL1, and the pca gene cluster is in the chromosome. The
particularity of the pht and pca gene cluster is
determined by homology comparison analysis with reported
PAEs-degradation gene clusters. The plasmid was successfully eliminated
by using SDS from strain ZJUTW, and the plasmid elimination the strain
ZJUTW could not grow in BSM with DBP was as the sole carbon source. The
result demonstrates that plasmid pQL1 is critical for the strain ZJUTW
to degrade DBP. To our best knowledge,
we
proposed a new complete metabolic pathway from DBP to Acetyl-CoA in the
genus Arthrobacter for the first time.