The performance of mNGS and standard procedures for detecting Aspergillosis
8 of 21 fungal pneumonia are Aspergillus pneumonia. Of the 8 cases, 7 cases were positive for mNGS, 5 cases were positive for Galactomannan antigen detection (GM test). 2 cases were positive for pathology, and only 1 case was positive for routine culture. One case was positive for ROSE, typical septate hyphae with sharp-angled bifurcation could be found with ROSE (Fig. 3A). Although the sensitivity of microbial culture was very low, the case missed by mNGS happened to be detected by microbial culture, and GM test also was positive (Table 2).