RORC and FOXP3 mRNA expression in thymoma
The relative expression level ofRORC mRNA in thymoma from the MG group was 2.44±1.59, which was higher than that from the Tm group (P <0.001). The relative expression level of FOXP3 mRNA in thymoma from MG group was 0.66±0.37, which was lower than that from the Tm group (P <0.001). The relative ratio ofRORC /FOXP3 in the MG group (4.29±2.84) was higher than that from the Tm group (P <0.001). (Fig. 5).
Cytokines ELISA assay in Thy0517culture medium
The contents of IL-6, IL-21 and TGF-β were higher in the culture medium of ty0517 cells, and the contents of IL-6 and IL-21 increased with days, while TGF-β decreased gradually. The contents of IL-1β, IL-4 and IL-23 were lower, and the changes were not significant with days. IL-12 was not detected in cell culture medium (Fig. 6)