The effect of ty0517 cells on the differentiation of human CD4 + T lymphocytes
After three days of coculture of thy0517 cells and CD4+ T cells, frequencies of Th17 and Tregs were analyzed by flow cytometry. The results showed that thy0517 cells could increase the percentage of Th17 cells (0.66 ± 0.07 vs 1.14 ± 0.08), and inhibit Tregs (3.10 ± 0.22 vs 2.41 ± 0.39). With the increase of PHA, the Th17 cells increased, while the increase of Tregs was not significant (Table 3 and Figure 7).