loading page

B cell epitope designing and their docking with ACE2 receptor for peptide based subunit vaccine against SARS-CoV-2: An antigen informatics approach
  • +1
  • Aditya Agrawal,
  • Mamta Pathak,
  • Shailesh PatelOrcid,
  • Vishal Rai
Aditya Agrawal
ICAR-Indian Veterinary Research Institute
Author Profile
Mamta Pathak
ICAR-Indian Veterinary Research Institute
Author Profile
Shailesh Patel
Orcid
ICAR-Indian Veterinary Research Institute
Author Profile
Vishal Rai
ICAR-Indian Veterinary Research Institute
Author Profile

Peer review status:IN REVISION

25 Mar 2020Submitted to PROTEINS: Structure, Function, and Bioinformatics
26 Mar 2020Submission Checks Completed
26 Mar 2020Assigned to Editor
06 Apr 2020Reviewer(s) Assigned
24 Apr 2020Review(s) Completed, Editorial Evaluation Pending
29 Apr 2020Editorial Decision: Revise Major
07 Aug 20201st Revision Received
10 Aug 2020Assigned to Editor
10 Aug 2020Submission Checks Completed

Abstract

Emerging pathogens have been an eternal threat to mankind. In a series of pandemics caused by notorious coronaviruses, a newly emerged virus is creating panic among world population. Originating in late December, 2019 from Wuhan, China, the SARS-CoV-2 is spreading its terror throughout the world. The virus is rapidly transmitted due to unavailability of effective theranostics. In this study, multiple sequence alignment of SARS-CoV-2 spike protein was done and four novel inserts were found in comparison to SARS-CoV. Using computational informatics tool viz. IEDB B-cell epitopes prediction tool, B-cell immunogenic epitopes of SARS-CoV-2 spike protein was predicted. Five novel potent epitopes i.e. ‘71SGTNGTKRFDN81, 247SYLTPG252, 634RVYST638, 675QTQTNSPRRARSV687 and 1054QSAPH1058 were selected as antigenic determinants and their docking with the 3D structure of ACE2 receptor was delineated. The quantum of information generated by this study will prove beneficial for development of effective therapeutics, diagnostics and multiepitopic vaccines to combat this ongoing menace.