Preparation of Ophthalmic Solutions
JV-GL1 isopropyl ester (and de-esterified JV-GL1) were supplied by
JeniVision Inc. Irvine, CA, USA). Butaprost was purchased from Cayman
Chemical Co. (Ann Arbor, MI, USA). Both drugs were dissolved in 100%
EtOH and made up to 0.01% in vehicle, composed of 0.2 µm sterile
filtered PBS, 0.2% DMSO and 1% polysorbate 80.