Figure 1. A) Effect of JV-GL1 and butaprost in normotensive Ptger2+/+ and Ptger2-/- mice. Three hours after single topical applications of 0.01% JV-GL1 or 0.01% butaprost to Ptger2+/+ mice, ΔIOP decreased to -3.1 [-4.1, -2.1] mmHg and -2.4 [-4.2, -0.6] mmHg respectively, returning to -0.002 [-1.0, 1.0] mmHg and 0.6 [-0.5, 1.5] mmHg by 24 hours. No effect was observed in Ptger2-/- mice after 3 hours (0.8 [-0.4, 2.0] mmHg vs 0.4 [-0.7, 1.5] mmHg, for JV-GL1 and butaprost respectively. Error bars are 95% confidence intervals. ΔIOP represents the difference in IOP between the treated eye relative to the contralateral untreated eye. B) Acute effect of 100 nM de-esterified JV-GL1 on outflow facility of enucleated C57BL/6J mouse eyes was not significantly different relative to contralateral vehicle perfused control eyes (-6% [-22, 13], P =0.4, n=5 pairs). Each data point shows the relative difference in reference facility\(\text{\ C}_{r}\), between paired eyes comparing the treated with respect to the untreated control eye. The error bars represent the 95% confidence intervals on the relative difference in\(\text{\ C}_{r}\). The light shaded region represents the best estimate of the sample distribution, with the geometric mean and two-sigma limits represented by the thick and thin horizontal white lines respectively. Dark central bands within the distribution represent the 95% CI on the estimated mean.