Molecular data
Molecular changes produced by genotype and AMPH exposure alone as well
as by their combination were analyzed using a two-way ANOVA, with the
factors genotype and type of AMPH access as independent variables. When
dictated by relevant interaction terms, Tukey’s multiple comparisons
test was used to characterize differences among individual groups of
rats. To enable visual comparisons across genotypes with different
degrees of expression of glutamatergic molecular determinants, values
are presented as percentage of the AMPH -naïve SERT+/+control rats that were not exposed to either AMPH ShA or LgA. Subjects
were eliminated from the final dataset if their data deviated from the
mean by 2 SDs. Prism 6.0 (GraphPad) was used to analyze all the data.
Data are shown as mean ± S.E.M and as % of baseline to control for
unwanted sources of variation. Significance for all tests was assumed atp < 0.05.